SmartCheck ladders are available in 3 size ranges - 50bp, 100bp, and 1kb - to cover most common applications.
Ready to use formulation includes buffer and tracking dye
500ul suitable for 100 lanes (5ul per lane)
High intensity reference bands
Ultra pure production allows for economical ambient shipping
Technical Details
Protocol: Briefly vortex the tube and use 5µl per lane. Additional loading buffer is not required.
Concentration: 0.1 mg/ml
Tracking dye: bromophenol blue and xylene cyanol
Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol
Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.
Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.
Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.
