Premixed 2X "hot start" PCR components for convenience and reproducibility
NovaTaq™ Hot Start Master Mix provides a ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2. The Master Mix simplifies the set-up for PCR and offers advantages of time savings, consistency, and minimal risk of contamination. In addition to the Master Mix, the kit includes PCR Grade Water and MgCl2 for optimizing Mg2+ concentrations. Simply add the NovaTaq Hot Start Master Mix to an equal volume containing DNA template, primers, and, if desired, additional MgCl2. The final diluted reaction contains 1.25 U of NovaTaq Hot Start DNA Polymerase per 50 µl. The two NovaTaq Hot Start Master Mix Kit sizes provide sufficient components for 200 or 1000 standard 50-µl amplification reactions.
