Sequential isolation of both DNA and RNA from the same FFPE sample using magnetic beads.
Mag-Bind® FFPE DNA/RNA 96 Kit is designed for sequentially isolating DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample, eliminating the use of hazardous xylene.
The specially formulated buffers reverse cross-linking without the need for overnight digestion, resulting in high-yielding, high-quality nucleic acids. The isolation protocol allows for the extraction of DNA and RNA in separate eluates from the same sample for a comprehensive analysis of both nucleic acids. Purified DNA and RNA are suitable for a variety of downstream applications, including SNP analysis, sequencing, and genotyping.
Mag-Bind® system is fully automatable on Hamilton Microlab® STAR™, Tecan Freedom Evo®, Thermo Fisher Scientific KingFisher® Flex Purification System, and other open-ended workstations.
