Western ReProbe™ is a single component system, specifically formulated to dissociate and remove antibodies from membrane bound proteins without destroying the antigenic binding affinity and does not use denaturants, SDS or boiling. Western ReProbe™ allows you the ability to reuse your Western blots.
Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1).
Western ReProbe™ is not recommended for stripping color producing Western blots that use substrates such as TMB, chloronapthol and DAB.
